YWHAG基因CDS的克隆及其在梅山与杜洛克猪卵泡发育中的差异表达研究

doi:10.11843/j.issn.0366-6964.2014.03.021

Abstract

Abstract:

To detect the expression pattern of YWHAG(Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein，gamma polypeptide) gene in during porcine follicle development.The CDS(Coding sequence) of YWHAG gene was cloned from porcine follicular tissue.Semi-quantitative RT-PCR was used to analyze the tissue expression profiling of YWHAG genes，Furthermore the expression level of YWHAG in follicles with different diameter(L，M2，M1，S) from Duroc and Meishan were investigated by real-time fluorescence quantitative PCR.The results showed that：the length of CDS of porcine YWHAG was 780 bp，and it’s similarity with that of human,mouse，and cattle was 97.72%，96.68% and 98.57%，respectively.YWHAG mRNA was expressed in all tested tissues(Muscle，fat，heart，liver，spleen，lung，kidney，stomach，small intestine，ovarian，uterine，uterine horn，tubal，pituitary，corpus luteum，brain，the hypothalamus)，and especially high in fat，intestine and brain.Both in Duroc and Meishan swine，the expression of YWHAG mRNA in M1 and L follicles were significantly higher than M2 and S follicles(P<0.05).Moreover，The expression in S and M2 follicles from Meishan were significantly higher than that from Duroc(P<0.01)，while no significant difference in M1 and L follicle.In conclusion，YWHAG gene may be involved in the apoptosis of porcine follicular development process，resulting in differences of ovulation rate in swine.

CLC Number:

S828.2

LIU Li-juan，LU Hui-wen,YANG Fei，XU Meng-si，HUANG Tao，LI Da-quan. CDS Cloning of Porcine YWHAG Gene and It’s mRNA Expression Difference between Meishan and Duroc Swine with Follicle Developing[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 489-493.

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CDS Cloning of Porcine YWHAG Gene and It’s mRNA Expression Difference between Meishan and Duroc Swine with Follicle Developing

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